A new social gene in Dictyostelium discoideum, chtB
1 Department of Ecology and Evolutionary Biology, Rice University, Houston, TX 77005, USA
2 Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
3 Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX, 77030, USA
4 Biology Department, Washington University in St. Louis, St. Louis, MO, 63130, USA
5 Present address: Department of Zoology, University of Oxford, Oxford, OX1 3PS, UK
BMC Evolutionary Biology 2013, 13:4 doi:10.1186/1471-2148-13-4Published: 9 January 2013
Additional file 1: Figure S1:
Spore production in pure populations and in chimeras. In each experiment we plated 5x107 cells on a KK2 plate and allowed them to develop. After 24 hours the contents of the plates were collected, treated with detergent and the spores counted. The sporulation efficiency of chtB is not significantly different from AX4.
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Additional file 2: Figure S2:
Germination efficiency assay. Spores of chtB and AX4 strains were plated on SM plates in association with bacteria and the number of viable spores was inferred from the number of plaques formed. chtB produce a percentage of viable spores comparable to AX4.
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