Additional file 4.

Organisation of the ventral nervous system in the tardigrade Macrobiotus cf. harmsworthi. RFamide-like immunoreactivity (glow mode) and DNA labelling (light blue); confocal micrographs; anterior is up in all images. (A) Entire specimen in ventral view. Asterisks indicate the position of the interpedal commissures. (B–D) Details of the posterior end showing the position of two RFamide-like immunoreactive somata associated with cloacal nerves (arrows). Abbreviations: cl, cloacal nerve; cn, connectives; ga1–ga4, trunk ganglia 1 to 4; ic, inner connective; le1–le4, walking legs 1 to 4; pg, peripheral ganglion; pn, peripheral nerve. Scale bars: 20 μm (A) and 10 μm (B–D).

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Mayer et al. BMC Evolutionary Biology 2013 13:230   doi:10.1186/1471-2148-13-230