Table 1

Heterandria formosa collection sites, sample sizes and genetic diversity measures
Population (specimen no. code†) No. Locality Latitude (° N) Longitude (° W) N Cytb haps. Study region
Bayou Nezpique (BNE x LA) 1 Bayou Nezpique, south of I-10, ~30 km east of Lake Charles, Cameron Par., LA 30.245 -92.627 2 22 WCP
Lake Pontchartrain (PON x LA) 2 Lake Pontchartrain, LA 30.189 -90.101 1 31 WCP
Bogue Chitto (BOG x LA) 3 Bogue Chitto R. overflow pool along LA Hwy 21 south of Sun, St. Tammany Par., LA 30.630 -89.897 2 30 WCP
Porter's River (POR x LA) 4 Porter's R. at Davis Landing Rd., St. Tammany Par., LA 30.318 -89.728 2 40, 41 WCP
Wolf Creek Swamp (WCMS x ) 5 Wolf Cr. swamp at Belle Ferry Rd., Harrison Co., MS 30.387 -89.197 2 42 WCP
Alabama River trib. (ALA x AL) 6 Unnamed trib. to Alabama R. (Bailey Cr. origin), Wilcox Co., AL 31.981 -87.504 2
Five Points (FIV x FL) 7 Unnamed trib. at Five Points, Tate's Hell State Forest, Franklin Co., FL 29.893 -84.760 9 17, 29 FL
Crooked River (CRO x FL) 8 Crooked R. at Tate's Hell State Forest, Franklin Co., FL 29.893 -84.731 10 17 FL
Ochlockonee River (OCH x FL) 9 Ochlockonee R. at Blountstown Hwy, Leon Co., FL 30.388 -84.665 2 1, 37 FL
Womack Creek (WOM x FL) 10 Womack Cr. at Apalachicola National Forest, Franklin Co., FL 30.009 -84.608 9 1, 3, 11-14 FL
Moore Lake (MOO x FL) 11 Moore Lake at Apalachicola National Forest, Leon Co., FL 30.392 -84.407 10 24 FL
Hill Swale (HIL x FL) 12 Hill Swale, just north of Ochlockonee Bay, Wakulla Co., FL 29.100 -84.406 10 25, 26 FL
Trout Pond (TRO x FL) 13 Trout Pond at Apalachicola National Forest, Leon Co., FL 30.335 -84.385 10 3 FL
Cessna Pond (CES x FL) 14 Cessna Pond at Apalachicola National Forest, Leon Co., FL 30.374 -84.360 9 3, 28 FL
Little Lake (LIT x FL) 15 Little Lake Jackson, west of Hwy 27, northwest Tallahassee, Leon Co., FL 30.526 -84.359 6 2 FL
Wakulla Springs (WAK x FL) 16 Wakulla Springs at Edward Ball Wakulla Springs State Park, Wakulla Co., FL 30.235 -84.302 10 7, 9-10, 20 FL
Lake Iamonia (IAM x FL) 17 Lake Iamonia at CR 155, Leon Co., FL 30.627 -84.291 10 1 FL
Shepherd Spring (SHE x FL) 18 Shepherd Spring at St. Marks National Wildlife Refuge, Wakulla Co., FL 30.123 -84.281 10 9-10, 27 FL
McBride Slough (MCB x FL) 19 McBride Slough at Bloxham Cutoff Rd., Wakulla Co., FL 30.239 -84.270 12 9-10, 43-44 FL
Lake Overstreet (LOV x FL) 20 Lake Overstreet at Alfred B. Maclay Gardens, Leon Co., FL 30.528 -84.257 10 1, 23 FL
Newport Sulphur Spring (NEW x FL) 21 Newport Sulphur Spring, Wakulla Co., FL 30.207 -84.179 10 2, 9, 15, 18 FL
Natural Bridge (NAT x FL) 22 Natural Bridge at Natural Bridge Rd., Wakulla Co., FL 30.284 -84.152 9 2, 18-19 FL
Gambo Bayou (GAM x FL) 23 Gambo Bayou at Lighthouse Rd. west of Stony Bayou Pool, Wakulla Co., FL 30.122 -84.148 9 9, 15 FL
Tram Road (TRA x FL) 24 Tram Rd., 2 km east of Plum Orchard, Wakulla Co., FL 30.140 -84.121 9 9, 15-16 FL
Wacissa River (WAC x FL) 25 Wacissa R., just north of Aucilla Wildlife Management Area, Jefferson Co., FL 30.341 -83.992 10 5-8 FL
Buggs Creek borrow pit (BUG x FL) 26 Buggs Creek barrow pit, FL 30.477 -83.844 2 35-36 FL
Wolf Creek (WOL x FL) 27 Wolf Creek at US 90, Jefferson Co., FL 30.532 -83.812 1 39 FL
Mckey Park (MCK x GA) 28 Mckey Park, Loundes Co., GA 30.864 -83.290 2 45 FL
Bevil Creek (BEV x GA) 29 Bevil Creek at Browns pond Loch Laurel Rd., GA 30.720 -83.244 2 3, 38 FL
Robinson Creek (ROB x FL) 30 Robinson Cr. at CR 131, Columbia Co., FL 30.321 -82.662 3 22 FL
Ichetucknee blue hole (ICH x FL) 31 Blue hole, Ichetucknee State Park, Columbia Co., FL 29.980 -82.758 2 32-33 FL
Hillsborough River (HIR x FL) 32 Hillsborough R. at Tampa, Hillsborough Co., FL 27.957 -82.465 11 22, 47 FL
River Styx (RIS x FL) 33 River Styx at CR 346, Alachua Co., FL 29.517 -82.222 2 FL
Saint Johns River (SJR2 x ) 34 Unnamed trib. of the St. Johns R., FL 3 22 FL
Newnan's Lake (NEWn x FL) 35 Newnan's Lake at southeast 16th Ave. boat ramp, Alachua Co., FL 29.637 -82.200 1 3 FL
Everglades (EVE x FL) 36 Everglades drainage, Dade Co., FL 25.552 -80.564 1 30 FL
Bahama Swamp (BAH x SC) 37 Bahama Swamp off Kato Bay Rd., Jasper Co., SC 32.356 -81.044 2 34 ACP
Edisto River (CRO x SC) 38 Edisto River, SC 33.085 -80.603 2 4, 46 ACP
Back River (BAC x SC) 39 Back R. at SSR 503, east of Goose Creek, edge of US Navy station, SC 32.967 -79.938 2 22 ACP
Cooper River trib. (WAD1 x SC) 40 Trib. to Cooper River at Wadboo, Berkeley Co., SC 33.201 -79.927 1 ACP
Waccamaw River (WAR1 x SC) 41 Waccamaw River, SC 33.474 -79.188 1 ACP
Trib. to Waccamaw River (WAR2 x SC) 42 Unnamed trib. to Waccamaw River, SC 33.506 -79.181 1 4 ACP
Lumber River (LUM x SC) 43 Lumber River, SC 33.661 -79.153 1 4 ACP
Cane Branch (CAN x SC) 44 Cane Branch on farm road at Francis Marion National Forest, Berkeley Co., SC 33.185 -79.527 1 21 ACP

Site names (numbers, localities, geographical coordinates), sample sizes (N), regional groups (Figure 1), and cytb haplotypes are indicated. Abbreviations: AL Alabama, Ave avenue, Co County, Cr Creek, CR County Road, FL Florida, GA Georgia, Hwy highway, MS Mississippi, Par Parish, R River, Rd Road, SC South Carolina, Trib tributary.

x-variables in codes range 1-12, representing each individual sequenced for this study.

Bagley et al.

Bagley et al. BMC Evolutionary Biology 2013 13:223   doi:10.1186/1471-2148-13-223

Open Data