Additional file 5.
Comparison of the expression patterns of the Dlx genes in the adult olfactory epithelium of E. burgeri. Haematoxylin- and eosin-stained sections (A) were obtained from the same level as the sections used for in situ hybridization with DIG-labelled probes for EbDlx1/4/6A (B), EbDlx1/4/6B (C), EbDlx1/4/6C (D), EbDlx2/3/5A (E), EbDlx2/3/5B (F), EbDlx2/3/5C (G), and EbDlxΨ (H). The sections for in situ hybridization were counterstained with ISH Red. All the genes were expressed in the olfactory epithelium. EbDlx1/4/6A, EbDlx2/3/5B, and EbDlx2/3/5C were detected in a few cells across the entire region of the olfactory epithelium. Strongly EbDlx1/4/6C-positive cells tended to be located at the levels of the non-basal olfactory epithelium cells. EbDlx2/3/5A showed strong expression in the basal cells. Subtle expression of EbDlx1/4/6B was detected in the entire region of the olfactory epithelium. EbDlxΨ showed a broad expression pattern in the basal cells and olfactory epithelium. The layers in which the probe signals were detected are indicated by brackets on the left side of the panels (B–H). Abbreviations: bc, basal cells; oe, olfactory epithelium. Scale bar, 100 μm.
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Fujimoto et al. BMC Evolutionary Biology 2013 13:15 doi:10.1186/1471-2148-13-15