Additional file 3.
The expression patterns of the Dlx genes in middle- and late-pharyngula embryos of E. burgeri. The sections of the middle-pharyngula embryos (A–H’) and the late-pharyngula embryos (I–X’) were derived from the embryos in Figure 1. Higher magnification of cranial cartilaginous tissues (trab and d.l.b) and inner ear in the late-pharyngeal embryos are shown in the third (J’–P’) and fifth rows (Q’–X’), respectively. Haematoxylin- and eosin-stained sections (A, I, I’, Q, and Q’) were obtained from the same level as the sections used for in situ hybridization with DIG-labelled probes for EbDlx1/4/6A (B, J, J’, R, and R’), EbDlx1/4/6B (C, K, K’, S, and S’), EbDlx1/4/6C (D, L, L’, T, and T’), EbDlx2/3/5A (E, M, M’, U, and U’), EbDlx2/3/5B (F, N, N’, V, and V’), EbDlx2/3/5C (G, O, O’, W, and W’), and EbDlxΨ (H, P, P’, X, and X’). The sections for in situ hybridization were counterstained with ISH Red. The signals from the probes in the mesenchymal cells (B, C, G, and O) and the cartilaginous primordium (N’) are indicated by arrowheads. Abbreviations: aud, auditory capsule; aud. c, auditory cartilage; c. bas, basal cartilage; coe, coelom; hb, hindbrain; d. l. b, dorsal longitudinal bar; ex. pq, extrapalato-quadrate; nchh, notochordal hook; nt, notochord; ot, otic vesicles; ph, pharynx; pp, pharyngeal pouch; t. cl, tendon of the clavatus muscle; t. vag, tendon of the vagina of the clavatus; trab, trabecular; v. br. b, ventral branchial bar; VII, facialis nerve; VIII, saccularis nerve. Scale bars, 100 μm.
Format: JPEG Size: 1.5MB Download file
Fujimoto et al. BMC Evolutionary Biology 2013 13:15 doi:10.1186/1471-2148-13-15