Expression patterns of the Dlx genes in the late-pharyngula embryo at the inner ear level. (A) Transverse view of a haematoxylin- and eosin-stained section. (B, C) Expression patterns of the Dlx genes. The sections for in situ hybridization were counterstained with ISH Red (B, C, E, F). (D–F) Higher magnification of the inner ear regions in A–C, respectively. EbDlx1/4/6B and EbDlx2/3/5B were weakly expressed in aud (E, F). The level of the section is indicated by an arrow in Figure 1B. Abbreviations: aud, auditory capsule; aud. c, auditory cartilage; c. bas, basal cartilage; d. l. b, dorsal longitudinal bar; ex. pq, extrapalato-quadrate; nchh, notochordal hook; ph, pharynx; t. cl, tendon of the clavatus muscle; t. vag, tendon of the vagina of the clavatus; v. br. b, ventral branchial bar. Scale bars, 100 μm (A); 10 μm (D).
Fujimoto et al. BMC Evolutionary Biology 2013 13:15 doi:10.1186/1471-2148-13-15