Expression patterns of the Dlx genes in the middle-pharyngula embryo at the otic placode level. (A) Transverse view of a haematoxylin- and eosin-stained section. (B–D) Expression patterns of the Dlx genes. DIG-labelled probes for EbDlx1/4/6A (B), EbDlx2/3/5B (C), and EbDlx2/3/5C (D) were detected in the ectomesenchymal cells (arrowheads). The sections used for in situ hybridization were counterstained with ISH Red (B–D). The level of the section is indicated by an arrow in Figure 1A. Abbreviations: coe, coelom; hb, hindbrain; nt, notochord; ot, otic vesicles; ph, pharynx; pp, pharyngeal pouch. Scale bar, 100 μm.
Fujimoto et al. BMC Evolutionary Biology 2013 13:15 doi:10.1186/1471-2148-13-15