Table 3

DAVID gene ontology functional annotation enrichment analysis in magnetic simulators
GBF ΔT O2 G level Affected genes (limma raw(FDR)) Behaviour responses Metamorphosis/morphogenesis/organogenesis Stress responses GO clusters observed also in control conditions
Reproductive/ mating/oviposition Sensorial/ hormonal/ odorant prot. Abiotic (heat, light & hypoxia) Biotic (defense & immune sys.) Insect cuticle proteins Cytoesqueleton and cell adhesion Proteolysis Red-Ox states/energy Hemo/ metals/ions binding
Mag No Yes 0g* UP 61(0) 4.5×10-2 3.8×10-2
0g* Down 62(0) 1.7×10-19 6.2×10-4
1g* UP 17(0)
1g* Down 25(0) 3.4×10-2
2g* UP 420(30) 6.4×10-2 8.8×10-3 2.1×10-4 1.3×10-2 1×10-2 5×10-8 6.7×10-4 3.1×10-3
2g* Down 328(42) 6.1×10-22 1.2×10-5 6.6×10-2 4.4×10-6 2.9×10-8
Yes Yes 0g* UP 635(458) 1.9×10-4 7.7×10-5 1.9×10-3 2.7×10-2 1.1×10-5 4.2×10-12 1.7×10-6
0g* Down 524(349) 2×10-2 2.3×10-3 3.4×10-2 1.5×10-2 2×10-25 2.9×10-3 7.2×10-11 4.7×10-5
1g* UP 350(196) 2.9×10-3 1×10-3 1.4×10-3 1.1×10-2 1.1×10-11 2.9×10-8
1g* Down 191(97) 2.5×10-18 2.7×10-2
2g* UP 208(7) 1.4×10-2 1.2×10-6 8.6×10-5
2g* Down 157(2) 2.3×10-15 6.8×10-2

The number of up- or down-regulated probesets in each GBF facility (first column) and condition (column 2 to 4) has been calculated and is shown in the fifth column (filtering with a p-value limma < 0.001). Relative enrichment analysis of these gene lists have been done, and the more frequent GO groups observed in experimental (columns 6 to 10), or 1g/environmental controls (columns 11 to 15) are included. Statistically meaningful p values are indicated in the table using italics and bold fonts to emphasize a greater level of enrichment in each particular GO group is observed. * indicates the presence of a high magnetic field.

Herranz et al.

Herranz et al. BMC Evolutionary Biology 2013 13:133   doi:10.1186/1471-2148-13-133

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