Table 2

DAVID gene ontology functional annotation enrichment analysis in mechanical simulators
GBF ΔT O2 G level Affected genes (limma raw(FDR)) Behaviour responses Metamorphosis/morphogenesis/organogenesis Stress responses GO clusters observed also in control conditions
Reproductive/ mating/oviposition Sensorial/ hormonal/ odorant prot. Abiotic (heat, light & hypoxia) Biotic (defense & immune sys.) Insect cuticle proteins Cytoesqueleton and cell adhesion Proteolysis Red-Ox states/energy Hemo/ metals/ions binding
RPM No No sim μg UP 218(0) 2.9×10-4 3.5×10-2 2.5×10-6
sim μg Down 117(0) 8.7×10-9 2×10-2 3.9×10-3
Yes No sim μg UP 28(0) 1.1×10-3
sim μg Down 17(0) 1.9×10-3
Yes Yes sim μg UP 20(0)
sim μg Down 21(0)
LDC No No 12g^ UP 85(0) 7×10-2 <10-14 4.2×10-3
12g^ Down 63(0) 1.7×10-3 1×10-3
6g^ UP 37(0) 2.4×10-5 1.1×10-2
6g^ Down 8(0) 1×10-2
Yes No 12g^ UP 873(185) 1.5×10-16 7.9×10-2 3×10-4 1.9×10-8
12g^ Down 614(72) 4.1×10-12 8.9×10-4 5.8×10-5 5.7×10-3
6g^ UP 288(0) 3.9×10-2 2×10-5 3.7×10-2
6g^ Down 118(0) 2.9×10-3 2.9×10-5 2.3×10-2 1.7×10-2 3.5×10-2
No Yes 12g^ UP >103(1557) 4.1×10-10 5.9×10-15 1.2×10-4
12g^ Down >103(1040) 4.7×10-3 8.4×10-22 5.1×10-7 3.4×10-3
6g^ UP >103(1359) 1.3×10-3 <10-15 7×10-2 3×10-6 1.5×10-16 5.3×10-4
6g^ Down 912(720) 7.4×10-4 6.8×10-5 3.1×10-6 1.7×10-2 1.9×10-9 5.4×10-11 5.8×10-7
Yes Yes 12g^ UP 608(6) 9×10-11 3×10-14 5.1×10-4 3.7×10-3 2.8×10-11 3.9×10-7
12g Down 334(0) 5.1×10-4 9.7×10-5
6g UP 419(1256) 4.9×10-17 6.1×10-2 3×10-2 7.8×10-2 3.7×10-2 8×10-2 7×10-6
6g Down 297(912) 9.6×10-12 2.5×10-4 7.7×10-8 7×10-4

The number of up- or down-regulated probesets in each GBF facility (first column) and condition (column 2 to 4) has been calculated and is shown in the fifth column (filtering with a p-value limma < 0.001, except for the LDC conditions (p-value FDR < 0.05 due to the large number of probesets detected)). Relative enrichment analysis of these gene lists have been done, and the more frequent GO groups observed in experimental (columns 6 to 10), or 1g/environmental controls (columns 11 to 15) are included. Statistically meaningful p values are indicated in the table using italics and bold fonts to emphasize a greater level of enrichment in each particular GO group is observed. ^ indicates the presence of mechanical/rotational disturbances.

Herranz et al.

Herranz et al. BMC Evolutionary Biology 2013 13:133   doi:10.1186/1471-2148-13-133

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