Table 4

Primer combinations of selective amplification
Primer combination MseI-Primer EcoRI-Primer Number of loci
1 MseI + CAG EcoRI + ACT + 6FAM™ 133
2 MseI + CAG EcoRI + ACA + VIC® 139
3 MseI + CAG EcoRI + AAG + NED™ 107
4 MseI + CTA EcoRI + AAG + PET® 68

All primers had 3 additional base pairs at the 3’end. EcoRI-Primers were also labeled by a fluorescent dye at the 5’end.

Scharnweber et al.

Scharnweber et al. BMC Evolutionary Biology 2013 13:132   doi:10.1186/1471-2148-13-132

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