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Open Access Highly Accessed Research article

Species richness, distribution and genetic diversity of Caenorhabditis nematodes in a remote tropical rainforest

Marie-Anne Félix1, Richard Jovelin2, Céline Ferrari345, Shery Han2, Young Ran Cho2, Erik C Andersen6, Asher D Cutter2 and Christian Braendle345*

  • * Corresponding author: Christian Braendle braendle@unice.fr

  • † Equal contributors

Author Affiliations

1 Institut de Biologie de l’Ecole Normale Supérieure, CNRS - ENS - INSERM, 46 rue d’Ulm, Paris cedex 05, 75230, France

2 Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks St, Toronto, ON, M5S 3B2, Canada

3 Institut de Biologie Valrose, CNRS, UMR7277, Parc Valrose, Nice cedex 02, 06108, France

4 INSERM, U1091, Nice cedex 02, 06108, France

5 University of Nice Sophia Antipolis, UFR Sciences, Nice cedex 02, 06108, France

6 Department of Ecology and Evolutionary Biology, Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA

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BMC Evolutionary Biology 2013, 13:10  doi:10.1186/1471-2148-13-10

Published: 12 January 2013

Abstract

Background

In stark contrast to the wealth of detail about C. elegans developmental biology and molecular genetics, biologists lack basic data for understanding the abundance and distribution of Caenorhabditis species in natural areas that are unperturbed by human influence.

Methods

Here we report the analysis of dense sampling from a small, remote site in the Amazonian rain forest of the Nouragues Natural Reserve in French Guiana.

Results

Sampling of rotting fruits and flowers revealed proliferating populations of Caenorhabditis, with up to three different species co-occurring within a single substrate sample, indicating remarkable overlap of local microhabitats. We isolated six species, representing the highest local species richness for Caenorhabditis encountered to date, including both tropically cosmopolitan and geographically restricted species not previously isolated elsewhere. We also documented the structure of within-species molecular diversity at multiple spatial scales, focusing on 57 C. briggsae isolates from French Guiana. Two distinct genetic subgroups co-occur even within a single fruit. However, the structure of C. briggsae population genetic diversity in French Guiana does not result from strong local patterning but instead presents a microcosm of global patterns of differentiation. We further integrate our observations with new data from nearly 50 additional recently collected C. briggsae isolates from both tropical and temperate regions of the world to re-evaluate local and global patterns of intraspecific diversity, providing the most comprehensive analysis to date for C. briggsae population structure across multiple spatial scales.

Conclusions

The abundance and species richness of Caenorhabditis nematodes is high in a Neotropical rainforest habitat that is subject to minimal human interference. Microhabitat preferences overlap for different local species, although global distributions include both cosmopolitan and geographically restricted groups. Local samples for the cosmopolitan C. briggsae mirror its pan-tropical patterns of intraspecific polymorphism. It remains an important challenge to decipher what drives Caenorhabditis distributions and diversity within and between species.

Keywords:
Caenorhabditis; Species richness; Population structure; C. briggsae; Nucleotide diversity