COI-based species delimitation analysis using the method of Pons et al. (2006). The Neotropical Troidini ultrametric tree obtained with PATHd8 shows clusters of specimens recognized as putative species. Genetic lineages recognized as putative species are highlighted in red and separated by longer black branches. The top corner graph shows the lineages-through-time plot based on the ultrametric tree. The sudden increase in branching rate, indicated by a red line, corresponds to the shift from interspecific (orange rectangle) to intraspecific (yellow rectangle) lineage branching. The vertical bars at the end of the branches group all sequences within each significant cluster, labelled by a provisional species name.
Condamine et al. BMC Evolutionary Biology 2012 12:82 doi:10.1186/1471-2148-12-82