C. elegans nhr-111 and nhr-239 are expressed genes. The nhr-111 and nhr-239 genes and their expression patterns are shown. A. Schematic of the nhr-111 and nhr-239 genes showing the basic gene structure and point of fusion to GFP in expression constructs. B. Expression of the nhr-111 and nhr-239 genes over time as measured in qRT-PCR experiments. Arbitrary expression units are 2-ΔCtx1010. Error bars represent the standard deviation among replicates. Data from embryos (EMB) and larval L1, L2/L3, and L4 preparations of wild-type cDNA are shown. C. Expression of the nhr-111::gfp transgene in L1, L2 and adult (Ad) animals. The Z1 and Z4 gonad precursors in the L1 are labelled. The L2 image shows expression throughout the developing somatic gonad. The PVD neurons and ventral nerve cord (vnc) are indicated in the adult panels. Arrowhead identifies the location of a prominent dorsal neuron that appears to be sensory. D. Expression of the nhr-239::gfp transgene in a two-fold embryo (EMB) and adult (Ad) hermaphrodites. Individual neuron pairs are identified by lower case letters: a, pair of pharyngeal neurons; b, pair of mid-lateral neurons or neuronal support cells; c, pair of dorsal neurons just posterior to the nerve ring with sensory dendritic processes (arrowhead). Due to the low level of nhr-239::gfp expression, brightness of the images in D was significantly increased relative to those in C.
Weber et al. BMC Evolutionary Biology 2012 12:81 doi:10.1186/1471-2148-12-81