Table 3

Summary of interspecific comparisons for regional Viburnum samples
Gene Region Species Aligned Length Variable characters Unique sequences % Max ID rate Max Mean (SD) > 1% > 2%
Central America and Mexico (17 species)
matK 14 719 10 3 21.29 0.0126 0.0022 (0.0044) 14.29 0
rbcL 12 491 3 4 33.33 0.0041 0.0015 (0.0018) 0 0
rpl32-trnL 15 876 22 8 53.33 0.0208 0.0038 (0.0057) 13.33 0.95
trnH-psbA 16 410 17 10 (12) 62.50 0.0265 0.0084 (0.0065) 35.83 8.33
trnK 12 1057 24 7 (8) 58.33 0.0202 0.0047 (0.0066) 16.67 6.06
nrITS 17 602 77 14 (15) 82.35 0.0829 0.0213 (0.0222) 56.62 33.09
rbcL + matK 10 1210 5 4 40.00 0.0033 0.0010 (0.0012) 0 0
rbcL + matK + trnH-psbA 11 1612 15 7 (9) 63.63 0.0070 0.0025 (0.0018) 0 0
rbcL + matK + nrITS 11 1807 61 10 91.00 0.0232 0.0068 (0.0060) 20.00 3.63
Japan (16 species)
matK 13 725 24 10 76.92% 0.0155 0.0081 (0.0040) 32.05 0
rbcL 13 491 8 6 46.15% 0.0144 0.0065 (0.0048) 35.90 0
rpl32-trnL 13 873 46 11 84.62% 0.0266 0.0162 (0.0073) 76.92 35.90
trnH-psbA 13 439 41 10 76.92% 0.0477 0.0176 (0.0099) 85.90 34.62
trnK 14 1057 36 10 71.43% 0.0172 0.0093 (0.0043) 48.35 0
nrITS 13 608 104 13 100.00% 0.0914 0.0551 (0.0213) 97.44 89.74
rbcL + matK 13 1216 32 11 84.62% 0.0125 0.0074 (0.0032) 12.82 0
rbcL + matK + trnH-psbA 12 1654 65 10 83.33% 0.0157 0.0097 (0.0041) 71.21 0
rbcL + matK + nrITS 12 1823 134 12 100.00% 0.0372 0.0230 (0.0089) 86.36 69.70

For the Viburnum species of Mexico and Central America and for Japan, the number of species analyzed, the aligned sequence length, the number of variable characters, the number of unique sequences with gaps treated as missing data (and with gaps treated as a fifth state), and the maximum number of species that can be identified by the data (Max ID rate = Identical sequences/total number of species) are reported. Summary statistics of genetic distances using a Kimura 2-parameter (K2P) model include: maximum genetic distance (max), mean interspecific distance (mean) with standard deviation (SD), and the proportion of comparisons of genetic distances greater than 1% (>1%) and greater than 2% (>2%).

Clement and Donoghue

Clement and Donoghue BMC Evolutionary Biology 2012 12:73   doi:10.1186/1471-2148-12-73

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