Perforin evolved from a gene duplication of MPEG1, followed by a complex pattern of gene gain and loss within Euteleostomi
1 Department of Biochemistry and Molecular Biology, Monash University, Clayton, Melbourne, VIC, 3800, Australia
2 The ARC Centre of Excellence in Structural and Functional Microbial Genomics, Monash University, Melbourne, VIC, 3800, Australia
3 Department of Microbiology, Monash University, Melbourne, VIC, 3800, Australia
4 Cancer Immunology Program, Peter MacCallum Cancer Centre, St Andrew’s Place, East Melbourne, VIC, 3002, Australia
5 Department of Microbiology and Immunology, The University of Melbourne, Parkville, VIC, 3010, Australia
BMC Evolutionary Biology 2012, 12:59 doi:10.1186/1471-2148-12-59Published: 2 May 2012
Additional file 1 :
Table S1. List of accessions used in this study.
Format: JPEG Size: 385KB Download file
Additional file 2 :
Figure S1. Manually annotated perforin protein sequences used in this study.
Format: FAS Size: 18KB Download file
Additional file 3 :
Figure S2. Platypus contig assembly by trace archive BLAST. Contigs are shown in blue with arrows indicating their relative 5’–3’ orientation. Linking BACs and fosmid clones are shown in grey and white respectively, with red regions showing where clone end sequences match contigs. Genes are shown as arrowheads with gene symbols above.
Format: FAS Size: 4KB Download file
Additional file 4 :
Figure S3. Alignment of fish perforin proteins for figure5tree.
Format: FAS Size: 17KB Download file