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Open Access Highly Accessed Research article

Molecular evolution of Drosophila Sex-lethal and related sex determining genes

Charles Mullon12, Andrew Pomiankowski12 and Max Reuter1*

Author Affiliations

1 Department of Genetics, Environment and Evolution, University College London, Darwin Building, Gower Street, London WC1E 6BT, UK

2 Centre of Mathematics and Physics in the Life Sciences and Experimental Biology, University College London, Physics Building, Gower Street, London WC1E 6BT, UK

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BMC Evolutionary Biology 2012, 12:5  doi:10.1186/1471-2148-12-5

Published: 14 January 2012

Additional files

Additional file 1:

Figure S1. Alignment of Sex-lethal of Drosophila species, the Tephritidae and M. domestica. Alignment used for analyses of Sex-lethal including sequences from Drosophila species, the Tephritidae and Musca domestica. The alignment is shown translated into amino acids. Sites under relaxed selection in the Tephritidae and Musca are indicated by a "1" in the line "Clade R ω = 1", those under positive selection on the basal brach leading to Drosophila Sex-lethal are indicated by a "*" in the line "Clade Droso ω > 1". These site-specific results are based on Bayes Empirical Bayes analyses mentioned in the main text. The RRM domains of the protein are also shown.

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Additional file 2:

Table S1. Maximum likelihood models of selection on Sxl in Drosophila, the Tephritidae and M. domestica sequences.

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Additional file 3:

Figure S2. Alignment of Sex-lethal of Drosophila species and the Tephritidae only, and ssx of Drosophila. Alignment used for analyses of Sex-lethal and ssx including sequences from Drosophila species and the Tephritidae. The alignment is shown translated into amino acids. Sites under relaxed selection in ssx (or CG3056) are indicated by a "1" in the line "Clade CG ω = 1", those under positive selection on the basal brach leading to ssx are indicated by a "*" in the line "Clade CG ω > 1". These site-specific results are based on Bayes Empirical Bayes analyses mentioned in the main text. The RRM domains of the protein are also shown.

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Additional file 4:

Table S2. Maximum likelihood ratio models for selection on Drosophila and tephritid Sxl and Drosophila ssx.

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Additional file 5:

Figure S3. Alignment of transformer of Drosophila species and the Tephritidae only. Alignment used for analyses of transformer including sequences from Drosophila species and the Tephritidae. The alignment is shown translated into amino acids. Sites under relaxed selection in the tephritid clade are indicated by a "1" in the line "Clade Teph ω = 1", those under relaxed selection in the Drosophila clade are indicated by a "1" in the line "Clade Droso ω = 1". These site-specific results are based on Bayes Empirical Bayes analyses mentioned in the main text. The domains that are conserved in the Tephritids but absent in Drosophila and longer that 10 AAs are from position 2 to 99, 215 to 249, 295 to 317 and 370 to 380 of A. serpentina.

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Additional file 6:

Table S3. Maximum likelihood models of selection on transformer in Drosophila and the Tephritidae.

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Additional file 7:

Figure S4. Alignment of doublesex of Drosophila species, the Tephritidae and M. domestica. Alignment used for analyses of doublesex including sequences from Drosophila species, the Tephritidae and Musca domestica. The alignment is shown translated into amino acids. Sites under positive selection on the basal brach leading to the tephritid clade are indicated by a "*" in the line "Clade Teph ω > 1", those under positive selection on the basal brach leading to the Drosophila clade are indicated by a "*" in the line "Clade Droso ω > 1". These site-specific results are based on Bayes Empirical Bayes analyses mentioned in the main text. Male- and female-specific domain of the protein are also shown.

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Additional file 8:

Table S4. Maximum likelihood models of selection on doublesex in Drosophila, the Tephritidae and M. domestica.

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Additional file 9:

Table S5. GI Accession numbers for sequences.

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