Figure 6.

(A) CHEF karyotypes of the founder population, 25°C. At left are the karyotypes of parental strains, S. cerevisiae CEN.PK, S. uvarum CBS7001, and their F1 interspecific hybrid. In lanes 3-24 are a set of random clones isolated from the common interspecific hybrid pool used to found all replicate populations. Green arrows indicate instances of karyotypic diversity (B) CHEF karyotypes after 500 generations of nitrogen limited, glucose sufficient culture with increasing temperature. 7 random clones were isolated from each experimental population. (C) CHEF karyotypes after 500 generations of nitrogen limited, glucose sufficient culture with increasing ethanol. 7 random clones were isolated from each experimental population. Three different S. cerevisiae reference markers are included: the parent CEN.PK, one from New England Biosystems (Sc NEB), and from Biorad (Sc Biorad).

Piotrowski et al. BMC Evolutionary Biology 2012 12:46   doi:10.1186/1471-2148-12-46
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