Figure 4.

Viability of parental species, F1 and founder interspecifc hybrids, and temperature-selected isolates in liquid culture following exposure to 48°C. (A) Inherent thermal tolerance. A sample of each culture was diluted and plated on 2% YPD every hour for 5 h. No viable S. uvarum cells were detected at the 1 h time-point, thus S. uvarum data are not presented. After 2 h survivorship of the selected isolates was greater than all other isolates (P < 0.05) (B) Induced thermal tolerance. Following overnight culture at 25°C, cells were incubated for 1 h at 37°C prior to exposure to 48°C. Samples of each culture were then diluted and plated every hour for 5 h. No viable S. uvarum cells were detected at the 1 h time-point, thus S. uvarum data and are not presented. After 2 h, survivorship of the selected isolates was greater than the founding hybrids (P < 0.05). Red lines and filled symbols represent the temperature-selected isolates. Experiments were performed in triplicate (Mean ± S.E.).

Piotrowski et al. BMC Evolutionary Biology 2012 12:46   doi:10.1186/1471-2148-12-46
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