Open Access Research article

Different selective pressures lead to different genomic outcomes as newly-formed hybrid yeasts evolve

Jeff S Piotrowski13, Saisubramanian Nagarajan23, Evgueny Kroll3, Alison Stanbery3, Kami E Chiotti3, Arthur L Kruckeberg4, Barbara Dunn5, Gavin Sherlock5 and Frank Rosenzweig3*

Author Affiliations

1 Chemical Genomics Research Group, RIKEN Advance Science Institute, Wako, Wako, Japan

2 School of Chemical and Biotechnology, SASTRA University, Tirumalaisamudram Thanjavur- 613401, Tamil Nadu, India

3 Division of Biological Sciences, The University of Montana, Missoula MT 59812, USA

4 DuPont Corporation, Wilmington DE 19880, USA

5 Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305-5120, USA

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BMC Evolutionary Biology 2012, 12:46  doi:10.1186/1471-2148-12-46

Published: 2 April 2012

Additional files

Additional file 1:

Table S1 Founding hybrid and selected isolates used in genetic and physiological experiments. Reference to each strain's CHEF karyotype is presented is column 2.

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Additional file 2:

Figure S1 Growth of evolved isolates with 8% ethanol supplementation. Culture density (A600) of parental, F1, founding and selected hybrid strains from each experimental population following 48 h growth in liquid, low-nitrogen, minimal medium at 25°C in medium, amended with 8% ethanol.

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Additional file 3:

Figure S2 Assay of Micafungin sensitivity of parental species, founding hybrids, temperature-selected hybrids, and ethanol selected hybrids. Growth of parental and temperature-selected hybrids in low-nitrogen, minimal medium at 25°C, supplemented with 150 nM of Micafungin. Presented is the growth (A600) relative to the solvent (DMSO) control of the 3 replicate cultures in stationary phase. Asterisks indicate that isolates in vessels A and C had significantly greater Micafungin resistance than all other isolates (P < 0.05, Mean ± S.E.).

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Additional file 4:

Figure S3 Changes in ploidy within experimentally selected populations. Cell populations were stained with SYTOX Green and sorted by flow cytometry as described. (A) S. cerevisiae haploid and S. cerevisiae/S. uvarum diploid. (B) Vessel A: 101gen (2 N), 157gen (mixed 2 N + 1 N), 224gen (1 N); (C) Vessel B: 115gen (2 N), 125gen (mixed 2 N + 1 N), 135gen (1 N); and (D) Vessel C: 43gen (2 N), 51gen (mixed 2 N + 1 N), 95gen (1 N).

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Additional file 5:

Figure S4 CHEF karyotypes in three experimental populations after 400 generations of nitrogen-limited, glucose-sufficient culture with increasing ethanol. At left are the karyotypes of parental strains, S. cerevisiae CEN.PK, S. uvarum CBS7001, and their F1 interspecific hybrid. 7 random clones were isolated from each experimental population. Green arrows indicate karyotypic variability in experimental populations. Medium ethanol content at 400 generations was 14%.

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