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Open Access Research article

Evolution, expansion and expression of the Kunitz/BPTI gene family associated with long-term blood feeding in Ixodes Scapularis

Shao-Xing Dai12, Ai-Di Zhang12 and Jing-Fei Huang12*

Author Affiliations

1 School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, P.R. China

2 State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, 32, Eastern Jiaochang Road, Kunming, Yunnan 650223, P.R. China

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BMC Evolutionary Biology 2012, 12:4  doi:10.1186/1471-2148-12-4

Published: 14 January 2012

Additional files

Additional file 1:

Figure S1. Two strategies for database searches of ticks Kunitz/BPTI proteins from NR database in NCBI.

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Additional file 2:

Table S1. Details of removed protein sequences.

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Additional file 3:

Table S2. Detailed information for all 303 sequences.

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Additional file 4:

Figure S2. Neighbor-joining (NJ) and maximum likelihood (ML) tree of single-domain Kunitz/BPTI proteins in Ixodes scapularis.

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Additional file 5:

Figure S3. Alignment of single-domain Kunitz/BPTI proteins in Ixodes scapularis.

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Additional file 6:

Figure S4. Neighbor-joining (NJ) and maximum likelihood (ML) tree of all single-domain Kunitz/BPTI proteins in ticks.

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Additional file 7:

Figure S5. Alignment of group II and group III Kunitz/BPTI protein sequences translated from ESTs in the Ixodes ricinus.

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Additional file 8:

Figure S6. Bayesian inference (MB) tree of single and multi-domain Kunitz/BPTI proteins in Ixodes scapularis.

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Additional file 9:

Table S3. Results of selection test for group II.

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Additional file 10:

Table S4. Results of selection test for group III.

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Additional file 11:

Table S5. Results of selection test for group I.

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Additional file 12:

S1. Results for assessing substitution saturation of the three groups.

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Additional file 13:

S2. Codon alignments of nucleotide sequences for three groups and script of the Perl program used in our study.

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Additional file 14:

Figure S7. Phylogeny of group I, II and III and for PAML analyze with dN and dS on each branch.

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Additional file 15:

Figure S8. Alignment of Ra-KLP related proteins in Metastriata ticks and group II proteins in Prostriata ticks.

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Additional file 16:

Table S6. Two search strategies have similar performance.

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Additional file 17:

Table S7. All sets of parameters used for sequence and phylogenetic analysis.

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Additional file 18:

Table S8. Details of the four cDNA libraries used for the expression analysis.

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