Table 3

ST-1 Genotypic evolution

Nucleotide

Gene

Change

Gene Function

End of phase I adaptation

ST-1_K-12

807

A

T- > C V50A

DNA replication

+*

+

1387

A

T- > C T210T

DNA replication

-

+

3869

F

G- > T V210F

Major coat protein

-

+

5540

H

A- > C D149G

Minor spike

+

+

All mutations from ST-1_K-12 were adaptive under conditions used for phase I of adaptation (R.H. Heineman, unpublished data)

*This mutation was only present in one of two sequenced isolates from this time point. The isolate with this additional change was used in all subsequent phenotypic assays

Chantranupong and Heineman BMC Evolutionary Biology 2012 12:37   doi:10.1186/1471-2148-12-37