Table 2

ΦX174 Genotypic Evolution

Nucleotide

Gene

Change

Gene Function

ΦX174C

ΦX174C#2

ΦX174ΔslyD

ΦX174slyDlow


756† ‡

D

T- > C F123L

External scaffolding protein

+

756† ‡

E

T- > C R63R

Lysis

+

873† ‡

J

G- > C G9A

DNA Packaging

+

930

J

C- > T A28V

DNA Packaging

+

1033

F

G- > C A11P

Major coat protein

+

1648

F

C- > T P216S

Major coat protein

+

3800

H

T- > C C868R

Minor spike protein

+

4706

A

G- > A S726S

DNA replication

4706

A*

G- > T208A

Nonessential

1735

F

A- > G A245A

Major coat protein

+

3339

H

G- > D137N

Minor spike protein

+

575§

D

G- > A T62T

External scaffolding protein

+

575§

E

G- > A R3H

Lysis

+

576

D

C- > T L63L

External scaffolding protein

+

+

576

E

C- > T R3R

Lysis

+

+

624§

D

G- > T A79S

External scaffolding protein

+

+

624§

E

G- > T L19F

Lysis

+

+


All changes were identical between two sequenced ΦX174C isolates

indicates mutation present in ΦX174 Af [42], one of the adapted lines used to start the adaptation

indicates mutation present in ΦX174pif- [43], the other adapted line with mutations available at the start of adaptation. All mutations in ΦX174C not present in ΦX174+ were sequenced in ΦX174pif-

£Also silent in context of change at 575

§Mutations previously found to compensate for slyD hosts [40]

Chantranupong and Heineman BMC Evolutionary Biology 2012 12:37   doi:10.1186/1471-2148-12-37

Open Data