Open Access Research article

Cryptic variation in an ecological indicator organism: mitochondrial and nuclear DNA sequence data confirm distinct lineages of Baetis harrisoni Barnard (Ephemeroptera: Baetidae) in southern Africa

Lyndall L Pereira-da-Conceicoa1*, Benjamin W Price123, Helen M Barber-James12, Nigel P Barker4, Ferdy C de Moor12 and Martin H Villet1

Author Affiliations

1 Department of Zoology and Entomology, Rhodes University, P.O. Box 94, Grahamstown, 6140, South Africa

2 Department of Freshwater Invertebrates, Albany Museum, Somerset Street, Grahamstown, 6140, South Africa

3 Department of Ecology & Evolutionary Biology, University of Connecticut, Storrs, 06269, CT, USA

4 Molecular Ecology & Systematics Group, Department of Botany, Rhodes University, P.O. Box 94, Grahamstown, 6140, South Africa

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BMC Evolutionary Biology 2012, 12:26  doi:10.1186/1471-2148-12-26

Published: 29 February 2012

Additional files

Additonal file 1:

Table S1. GenBank Accession numbers, samples and localities. Clade labels correspond to Figures 2 and 3. (‘-’ indicates failure to amplify; * = sequence obtained from GenBank; EC = Eastern Cape; KZN = KwaZulu-Natal; LIM = Limpopo; MPU = Mpumalanga; NW = North West; RSA = South Africa; WC = Western Cape).

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Additonal file 2:

Figure S1. Plot of the mean time to most recent common ancestor (TMRCA). Estimated using BEAST under differing tree priors and substitution rates. (A) mean COI substitution rate of 3.5% per MY; (B) mean COI substitution rate of 1.5% per MY. Error bars correspond to the estimated 95% HPD. Dashed lines denote approximate boundaries of geologic epochs: Oli. Oligocene; Mio. Miocene; Pli. Pliocene; Ple. Pleistocene. Node numbering corresponds to Figure 2.

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