Additional file 7.
Figure S4. Outbreeding rescued the fertility loss caused by stress at 28°C in Hsp83 mutants. (A) Experimental design for outbreeding of flies at 28°C. Flies from 7 isofemale lines (iso-1 to iso-7) for each genotype (wildtype Hsp83+/+ vs. mutant Hsp83P/P) were collected from adults eclosed in the second generation of inbreeding at 28°C for the Ivory Coast population (see the inbreeding design in Materials and methods and additional file 6). Virgin females from one line and males from another line with the same genotype were mated to establish between-line crosses. In addition, virgin females from one mutant line and males from a wild-type line were mated to set up between-genotype crosses. In total, seven between-line crosses for each genotype and seven between-genotype crosses were established. In each cross, four 3-day-old females and four 3-day-old males were mated in a vial. The eight flies in the vial were transferred to a new vial with fresh food every other day for 30 days. Adults eclosing from each vial were censused every other day until eclosion ceased. × indicates crossing. (B) female fecundity from crosses between lines and crosses between genotypes. Independent-sample t-tests were performed to calculate significance of fecundity difference between two crosses. Asterisks (*) indicate significant differences at p < 0.05 in these tests.
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Chen and Wagner BMC Evolutionary Biology 2012 12:25 doi:10.1186/1471-2148-12-25