Wild-type Hsp83 alleles can buffer cryptic deleterious variation caused by inbreeding. Each panel shows changes in female fecundity (vertical axes) over 3-4 generations (horizontal axes) of inbreeding in fly populations containing either Hsp83 mutant or wild-type alleles. Seven lines homogenous for Hsp83 wild-type (Hsp83+/+) and for Hsp83 mutant (Hsp83P/P), respectively, were isogenized from each of three populations Okayama, Tokyo, and the Ivory Coast. In each generation, four virgin females and four males of an isofemale line were crossed to reproduce the next generation. All offspring produced within 30 days were counted as a measurement of female fecundity. Error bars indicate one standard error of the mean for the 7 isofemale lines. Independent-sample t-tests were performed to calculate the significance of fecundity difference between generation one, on the one hand, and generations 2-4, on the other hand. Asterisks indicate significant differences in fecundity to generation one: at p < 0.05(*) and p < 0.01(**).
Chen and Wagner BMC Evolutionary Biology 2012 12:25 doi:10.1186/1471-2148-12-25