Open Access Research article

Analyses of expressed sequence tags in Neurospora reveal rapid evolution of genes associated with the early stages of sexual reproduction in fungi

Kristiina Nygren15, Andreas Wallberg2, Nicklas Samils1, Jason E Stajich3, Jeffrey P Townsend4, Magnus Karlsson1 and Hanna Johannesson5*

Author Affiliations

1 Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences, Box 7026, SE-75007, Uppsala, Sweden

2 Department of Medical Biochemistry and Microbiology, Uppsala University, Box 582, SE-751, Uppsala, Sweden

3 Department of Plant Pathology and Microbiology, University of California at Riverside, 900 University Avenue, Riverside, CA, 92521, USA

4 Department of Ecology and Evolutionary Biology, Yale University, 165 Prospect St., New Haven, CT, 06520-8106, USA

5 Department of Evolutionary Biology, Evolutionary Biology Centre, Uppsala University, Norbyvägen 18 D, SE-752 36, Uppsala, Sweden

For all author emails, please log on.

BMC Evolutionary Biology 2012, 12:229  doi:10.1186/1471-2148-12-229

Published: 27 November 2012

Additional files

Additional file 1: Table S1:

A list of rapidly evolving genes, and the branch(es) in which a dN/dS higher than mean are found.

Format: PDF Size: 43KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 2: Table S2:

Candidate genes used to test for positive selection, and ID of additional heterothallic Neurospora strains sequenced. (PDF 51 kb)

Format: PDF Size: 51KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 3: Figure S1:

Nucleotide and amino acid alignments of the three genes NCU03013, NCU06387, and NCU07311, showing which codons were found to evolve under positive selection. (PDF 13386 kb)

Format: PDF Size: 13.1MB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 4: Table S3:

Primers used for PCR amplification of candidate genes for test for positive selection. (PDF 34 kb)

Format: PDF Size: 34KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data