Table 2

Overview of GHF5 cellulase sequences generated in this study from plant parasitic nematodes belonging to the superfamily Hoplolaimoidea
Species name Individual* Forward primer (1) Reverse primer(1) Fragment length (bp) Fragment length after removal putative introns (bp) Gene name (2) GenBank Acc. No.
Meloidogyne ardenensis 1 CD1cF CD6cR 1228 727 Mard-eng-1 JN052024
Meloidogyne artiellia 1 CD1MelF CD6MelR 936 731 Mart-eng-1 JN052025
Meloidogyne ichinochei 1 CD2aF CD4aR 673 343 Mic-eng-1 JN052026
M. ichinochei 2 CD2aF CD4aR 673 343 Mic-eng-2 JN052027
Hirschmanniella gracilis 1 CD1aF CD6bR 950 728 Hgr-eng-1 JN052061
H. gracilis 1 CD1aF CD6bR 866 728 Hgr-eng-2 JN052062
H. gracilis 1 CD1aF CD6bR 950 728 Hgr-eng-3 JN052063
Hirschmanniella loofi 1 CD1bF ENG1R 282 255 Hl-eng-1 JN052057
H. loofi 1 CD1bF CD4aR 560 487 Hl-eng-2 JN052058
H. loofi 1 ENG1 CD6aR 983 461 Hl-eng-3 JN052059
H. loofi 1 ENG1 CD6aR 554 431 Hl-eng-4 JN052060
Pratylenchus crenatus 1 CD1PraFa CD6aR 820 721 Pcr-eng-1 JN052031
P. crenatus 2 CD1PraFb CD6PraRa 819 723 Pcr-eng-2 JN052030
P. crenatus 3 ENG1F CD6aR 543 449 Pcr-eng-3 JN052029
Pratylenchus neglectus 1 ENG1 CD6aR 508 452 Pn-eng-1 JN052032
P. neglectus 2 CD1PraFc CD6PraRa 791 735 Pn-eng-2 JN052033
P. neglectus 2 CD1PraFc CD6PraRa 789 733 Pn-eng-3 JN052034
Pratylenchus penetrans 1 CD2cF CD6aR 1514 588 Pp-eng-3 JN052035
P. penetrans 1 CD2cF CD6aR 695 587 Pp-eng-4 JN052036
P. penetrans 1 CD1bF CD4aR 739 484 Pp-eng-5 JN052037
P. penetrans 2 CD1PraFb CD6PraRb 841 733 Pp-eng-6 JN052038
Pratylenchus convalariae (3) 1 CD1PraFb CD6aR 874 739 Pcon-eng-1 JN052028
Pratylenchus pratensis 1 CD1bF ENG1R 256 256 Ppr-eng-1 JN052040
P. pratensis 1 CD2bF CD6dR 1124 592 Ppr-eng-2 JN052039
P. pratensis 1 CD2dF CD4aR 407 349 Ppr-eng-3 JN052041
P. pratensis 1 CD1aF CD4aR 489 489 Ppr-eng-4 JN052043
P. pratensis 1 ENG1 CD6aR 507 452 Ppr-eng-5 JN052042
P. pratensis 2 CD1PraFb CD6PraRa 801 750 Ppr-eng-6 JN052044
Pratylenchus thornei 1 CD2bF CD6dR 678 587 Pt-eng-1 JN052045
P. thornei 2 CD1PraFc CD6PraRb 824 733 Pt-eng-2 JN052046
Pratylenchus vulnus 1 CD2cF CD6aR 639 587 Pv-eng-1 JN052047
P. vulnus 1 CD2bF CD6dR 1001 614 Pv-eng-2 JN052048
P. vulnus 1 CD2bF CD6dR 639 587 Pv-eng-3 JN052049
P. vulnus 1 CD2aF CD4aR 349 349 Pv-eng-4 JN052050
P. vulnus 1 ENG1 CD6aR 958 449 Pv-eng-5 JN052051
P. vulnus 1 ENG1 CD6aR 604 312 Pv-eng-6 JN052052
P. vulnus 1 ENG1 CD6aR 505 452 Pv-eng-7 JN052053
P. vulnus 2 CD1aF CD6bR 1031 728 Pv-eng-8 JN052054
P. vulnus 3 CD1PraFb CD6aR 790 733 Pv-eng-9 JN052055
P. vulnus 3 CD1PraFb CD6PraRa 792 735 Pv-eng-10 JN052056
Globodera pallida 1 CD1aF CD4cR 1144 481 Gp-eng-1 JN052064
G. pallida 1 CD2aF CD6cR 852 589 Gp-eng-2 JN052065
G. pallida 1 CD2aF CD6cR 853 590 Gp-eng-3 JN052066
G. pallida 2 CDGp2F CD6cR 1561 709 Gp-eng-4 JN052067
G. pallida 2 CDGp2F CDGp8R 706 706 Gp-eng-5 JN052068

Overview of GHF5 cellulase sequences generated in this study from plant parasitic nematodes belonging to the superfamily Hoplolaimoidea. Single nematodes were used for the amplification of putative cellulase fragments. Occasionally, multiple fragments were amplified from an individual, such as in case of Pratylenchus neglectus 2 (Pn-eng-2 and −3). (1) Primers sequences are given in Table 1. (2) gene names in bold indicate fragments spanning almost the full catalytic domain (CD1 - CD6). (3) Small subunit ribosomal DNA data suggest that Pratylenchus convalariae is identical to P. penetrans[20].

Rybarczyk-Mydłowska et al.

Rybarczyk-Mydłowska et al. BMC Evolutionary Biology 2012 12:221   doi:10.1186/1471-2148-12-221

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