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Open Access Research article

Expansion of the gamma-gliadin gene family in Aegilops and Triticum

Svetlana V Goryunova12, Elma MJ Salentijn13, Nadejda N Chikida4, Elena Z Kochieva5, Ingrid M van der Meer36, Luud JWJ Gilissen36 and Marinus JM Smulders136*

Author affiliations

1 Wageningen UR Plant Breeding, Wageningen UR, P.O. Box 16, Wageningen, NL-6700 AA, The Netherlands

2 Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991, Russia

3 Plant Research International, Wageningen UR, P.O. Box 16, Wageningen, NL-6700 AA, The Netherlands

4 All-Russian Institute of Plant Industry, St. Petersburg, 190000, Russia

5 Bioengineering Center, Moscow, 117312, Russia

6 Allergy Consortium, Wageningen, The Netherlands

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Citation and License

BMC Evolutionary Biology 2012, 12:215  doi:10.1186/1471-2148-12-215

Published: 8 November 2012

Abstract

Background

The gamma-gliadins are considered to be the oldest of the gliadin family of storage proteins in Aegilops/Triticum. However, the expansion of this multigene family has not been studied in an evolutionary perspective.

Results

We have cloned 59 gamma-gliadin genes from Aegilops and Triticum species (Aegilops caudata L., Aegilops comosa Sm. in Sibth. & Sm., Aegilops mutica Boiss., Aegilops speltoides Tausch, Aegilops tauschii Coss., Aegilops umbellulata Zhuk., Aegilops uniaristata Vis., and Triticum monococcum L.) representing eight different genomes: Am, B/S, C, D, M, N, T and U. Overall, 15% of the sequences contained internal stop codons resulting in pseudogenes, but this percentage was variable among genomes, up to over 50% in Ae. umbellulata. The most common length of the deduced protein, including the signal peptide, was 302 amino acids, but the length varied from 215 to 362 amino acids, both obtained from Ae. speltoides. Most genes encoded proteins with eight cysteines. However, all Aegilops species had genes that encoded a gamma-gliadin protein of 302 amino acids with an additional cysteine. These conserved nine-cysteine gamma-gliadins may perform a specific function, possibly as chain terminators in gluten network formation in protein bodies during endosperm development. A phylogenetic analysis of gamma-gliadins derived from Aegilops and Triticum species and the related genera Lophopyrum, Crithopsis, and Dasypyrum showed six groups of genes. Most Aegilops species contained gamma-gliadin genes from several of these groups, which also included sequences from the genera Lophopyrum, Crithopsis, and Dasypyrum. Hordein and secalin sequences formed separate groups.

Conclusions

We present a model for the evolution of the gamma-gliadins from which we deduce that the most recent common ancestor (MRCA) of Aegilops/Triticum-Dasypyrum-Lophopyrum-Crithopsis already had four groups of gamma-gliadin sequences, presumably the result of two rounds of duplication of the locus.

Keywords:
Gamma-gliadin; Wheat; Triticum; Aegilops; Multigene family; Evolution