Figure 6.

Sequence and expression of Ame-mir-0005/mir-92b. (A) Sequence of the novel mir-0005 precursor, with the sequence reads aligned below and associated read counts. Mature miRNA and miRNA* sequences are shown surrounded by asterisks. Bracket-notation of secondary structure is shown and highlighted in red is the sequence with the highest read count. (B) The predicted pre-mir-0005 hairpin structure, with the most abundant read sequence highlighted in red. (C-F) Embryonic expression during honeybee embryogenesis of pri-Ame-mir-0005. (C) Lateral view of a stage 4 embryo. Pri-Ame-mir-0005 RNA expression was strongest in the posterior third of the embryo. (D) Higher magnification view of the boxed region in (C) showing nuclear dot staining but not in the adjacent region where only background staining was detected (no nuclear staining (NNS)). (E) Ventral view of a stage 9 embryo revealing staining in the amnion region of the embryo.