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Open Access Research article

Contrasted patterns of selective pressure in three recent paralogous gene pairs in the Medicago genus (L.)

Joan Ho-Huu1, Joëlle Ronfort1, Stéphane De Mita12, Thomas Bataillon3, Isabelle Hochu1, Audrey Weber1 and Nathalie Chantret1*

Author Affiliations

1 INRA - Institut National de la Recherche Agronomique, UMR AGAP, Montpellier, 34060, France

2 INRA - Institut National de la Recherche Agronomique, UMR IAM, Nancy, France

3 Bioinformatics Research Center (BiRC), Aarhus University, Aarhus, Denmark

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BMC Evolutionary Biology 2012, 12:195  doi:10.1186/1471-2148-12-195

Published: 1 October 2012

Abstract

Background

Gene duplications are a molecular mechanism potentially mediating generation of functional novelty. However, the probabilities of maintenance and functional divergence of duplicated genes are shaped by selective pressures acting on gene copies immediately after the duplication event. The ratio of non-synonymous to synonymous substitution rates in protein-coding sequences provides a means to investigate selective pressures based on genic sequences. Three molecular signatures can reveal early stages of functional divergence between gene copies: change in the level of purifying selection between paralogous genes, occurrence of positive selection, and transient relaxed purifying selection following gene duplication. We studied three pairs of genes that are known to be involved in an interaction with symbiotic bacteria and were recently duplicated in the history of the Medicago genus (Fabaceae). We sequenced two pairs of polygalacturonase genes (Pg11-Pg3 and Pg11a-Pg11c) and one pair of auxine transporter-like genes (Lax2-Lax4) in 17 species belonging to the Medicago genus, and sought for molecular signatures of differentiation between copies.

Results

Selective histories revealed by these three signatures of molecular differentiation were found to be markedly different between each pair of paralogs. We found sites under positive selection in the Pg11 paralogs while Pg3 has mainly evolved under purifying selection. The most recent paralogs examined Pg11a and Pg11c, are both undergoing positive selection and might be acquiring new functions. Lax2 and Lax4 paralogs are both under strong purifying selection, but still underwent a temporary relaxation of purifying selection immediately after duplication.

Conclusions

This study illustrates the variety of selective pressures undergone by duplicated genes and the effect of age of the duplication. We found that relaxation of selective constraints immediately after duplication might promote adaptive divergence.

Keywords:
Duplication; Medicago; Neofunctionalization; Subfunctionalization; Paralogs evolution