Open Access Research article

The diversity and evolution of chelicerate hemocyanins

Peter Rehm1, Christian Pick1, Janus Borner1, Jürgen Markl2 and Thorsten Burmester1*

Author Affiliations

1 Institute of Zoology and Zoological Museum, University of Hamburg, D-20146 Hamburg, Germany

2 Institute of Zoology, Johannes Gutenberg University Mainz, D-55099 Mainz, Germany

For all author emails, please log on.

BMC Evolutionary Biology 2012, 12:19  doi:10.1186/1471-2148-12-19

Published: 14 February 2012

Additional files

Additional file 1:

List of sequences used in this study. The accession numbers of the cDNA sequences are given, except (*), which has been derived by conventional protein sequencing. SU = subunit.

Format: DOC Size: 242KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 2:

Multiple sequence alignment of chelicerate, crustacean, myriapod and insect hemocyanins, and selected arthropod phenoloxidases.

Format: DOC Size: 437KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 3:

Molecular properties of chelicerate hemocyanin cDNA and the deduced amino acid. The asterisks (*) denote incomplete N-terminal sequences of P. imperator hemocyanins subunits, with 8 and 9 amino acids missing.

Format: DOC Size: 161KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 4:

Phylogenetic tree of the chelicerate hemocyanin subunits excluding AgoHcX. The numbers at the nodes represent Bayesian posterior probabilities estimated with the WAG model of amino acid substitution. The species abbreviations are: Aau, Androctonus australis; Ago, Acanthoscurria gomesiana; Cro, Carcinoscorpius rotundicauda; Csa, Cupiennius salei; Eba, Euphrynichus bacillifer; Eca, Eurypelma californicum; Esp, Endeis spinosa; Lpo, Limulus polyphemus; Mgi, Mastigoproctus giganteus; Nin, Nephila inaurata; Pin, Pandinus imperator; Ttr, Tachypleus tridentatus. See Additional file 1 for abbreviations of the proteins.

Format: DOC Size: 1.1MB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 5:

Divergence times of chelicerate hemocyanin subunit types (see Figure 2A). Rates across sites were modeled assuming a gamma distribution (Γ) or with a Dirichlet process (D). Divergence time priors were either uniform or modeled with a birth death process. Hard or soft bounds were applied. Divergence times are given in Ma.

Format: DOC Size: 54KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 6:

Divergence times of chelicerate taxa, as estimated from the hemocyanin sequences (see Figure 2B). Rates across sites were modeled assuming a gamma distribution (Γ) or with a Dirichlet process (D). Divergence time priors were either uniform or modeled with a birth death process. Hard or soft bounds were applied. Divergence times are given in Ma.

Format: DOC Size: 52KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data