Table 2

Characterized carbohydrate-active enzymes of family GH5 not yet classified into subfamilies
Description EC Assay Organism Accessions Modular structure Taxonomic class
β-mannanase A (ManA;CelA) 3.2.1.78 Caldanaerobius polysaccharolyticus KM-THCJ AAD09354 GH5 CBM16 CBM16 B-Firmicutes_Clostridia
endo-β-1,4-glucanase D (CelD; CelCCD; EGCCD; Ccel_0840) 3.2.1.4 Clostridium cellulolyticum H10 [B] BAA14354 ACL75216 GH5 CBM11 DOC B-Firmicutes_Clostridia
endo-β-1,4-glucanase/b-1,3:1,4-glucanase H (CelH) 3.2.1.4 3.2.1.73 Clostridium thermocellum NCIB 10682 AAA23225 GH26 GH5 CBM11 DOC B-Firmicutes_Clostridia
cellulase (EBI-244) 3.2.1.4 Desulfurococcaceae archaeon EBI-244 AEB53062 GH5 A-Crenarchaeota
endo-β-1,4-glucanase 3 (Cel3;Cel-3; Eg3; Fisuc_2230; FSU_2772) 3.2.1.4 Fibrobacter succinogenes subsp. succinogenes S85 AAA24893 ACX75816 ADL25000 GH5 B-Fibrobacteres_Acidobacteria group
Fisuc_2933/FSU_0196 AGM Fibrobacter succinogenes subsp. succinogenes S85 ACX76513 ADL26912 GH5 CBM4 B-Fibrobacteres_Acidobacteria group
Fisuc_1523/FSU_2005 MUC Fibrobacter succinogenes subsp. succinogenes S85 ACX75120 ADL26743 GH5 B-Fibrobacteres_Acidobacteria group
endoglucanase (CelA; lpg1918) 3.2.1.4 AHEC Legionella pneumophila subsp. pneumophila str. Philadelphia 1 AAU27988 GH5 B-Gammaproteobacteria
endo-β-1,4-glucanase 5B (Sde_2490) 3.2.1.4 Saccharophagus degradans 2-40 ABD81750 CBM6 GH5 B-Gammaproteobacteria
endo-β-1,4-glucanase 5E (Sde_2929) 3.2.1.4 Saccharophagus degradans 2-40 ABD82186 CBM6 CBM6 GH5 B-Gammaproteobacteria
endo-β-1,6-glucanase (Exg3; SPBC2D10.05) 3.2.1.75 Schizosaccharomyces pombe 972 h- CAA21163 NP_596224 GH5 E-Fungi
endo-β-1,4-glucanase (Cel5G) 3.2.1.4 uncultured bacterium ADD71777 GH5 B-environmental samples
SARM_0034/694713_55880/TW39 LIC CMC uncultured organism ADX05705 GH5 U-unclassified sequences
SARM_0047/1057205_158590/TW-15 CMC uncultured organism ADX05718 GH5 U-unclassified sequences
SARM_0086/0_06533/TW-18 PCW uncultured organism ADX05761 GH5 U-unclassified sequences
β-glucanase (RR.06; RR.06-1; BglC) BBG Cel5 LIC unidentified microorganism CAJ19140 GH5 U-unclassified sequences
β-glucanase (RR.10; RR.10-1) BBG unidentified microorganism CAJ19146 U-unclassified sequences

The active enzymes are tagged by their Enzyme Classification (EC) number (see Table 1) or by the significant positively assayed substrates that are: AEHC - AZCL-HE cellulose; AGM - AZCL-galactomannan; BBG – barley β-glucan; Cel5 – cellopentose; CMC - carboxymethyl cellulose; LIC –lichenan; MUC - 4-methylumbelliferyl-β-D-cellobioside; PCW – plant cell wall.

Aspeborg et al.

Aspeborg et al. BMC Evolutionary Biology 2012 12:186   doi:10.1186/1471-2148-12-186

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