Examples of modular GH5 proteins. (a) Diverse modular arrangements of putative monofunctional modular enzymes from subfamily GH5_8. (b) Same for putative bifunctional GH5 enzymes containing a subfamily GH5_8 module. (c) Other putative bifunctional enzymes containing at least a single GH5 module. (d) Selected examples of proteins containing GH5 modules having lost one or more catalytic residues. For a given protein, each GH5 module is identified by a number of fields separated by “|” indicating: (i) the organism, with 3 letters for the genre and either 5 letters for the species or full strain code; (ii) the GenBank protein accession; (iii) if attributed, the subfamily number or other information; (iv) EC numbers if available. These individual tags are analogous to what is found in Additional file 1: Figure S1. The module types and other protein segments present are: GHx_y – glycoside hydrolase family x subfamily y (pink); CEx – carbohydrate esterase module of family x (light brown); Cip21 – chitin-binding protein type 21 module with putative carbohydrate oxidative cleaving activity, formerly CBM33 (dark gray); CBMx – carbohydrate binding modules of family x (light green); FN3 – fibronectin type III modules (dark green); DOC – cellulosomal dockerin modules (light violet); EXPN – expansin modules (dark purple); signal peptides (purple); transmembrane segments (yellow); linkers (light blue); other regions (light grey).
Aspeborg et al. BMC Evolutionary Biology 2012 12:186 doi:10.1186/1471-2148-12-186