Table 5

All primers used for PCR amplification and sequencing in this study
Marker Primer name Forward primer sequence Annealing temperature (°C) Reference
ITS ITS1F 5’-CTTGGTCATTTAGAGGAAGTAA-3’ 55-60 [113]
ITS_Mel_F 5’- TGCTTTGGCGGRYCYYRRG-3’ 55-60 This study
ITS4 5’-TCC CCGCTTATTGATATGC-3’ 55-60 [114]
ITS4A 5’- CGCCGTTACTGGGGCAATCCCTG-3’ 55-60 [115]
ITS4 5’-TCCTCCGCTTATTGATATGC-3’ 55-60 [114]
LSU Al2R 5’-GCGAGTGAAGCGGCAACAGCTC3’ 55-60 [116]
LR3 5’-CCGTGTTTCAAGACGGG-3’ 55-60 Vilgalys unpublished
mtSSU mrSSU1 5’-AGCAGTGAGGAATATTGGTC-3’ 55-60 [117]
mrSSU3R 5’-ATGTGGCACGTCTATAGCCC-3’ 55-60 [117]
MCM7 Mcm7-709for 5’-ACIMGIGTITCVGAYGTHAARCC-3’ 56 [112]
Mcm7-1348rev 5’-GAYTTDGCIACICCIGGRTCWCCCAT-3’ 56 [112]
X_Mcm7_F 5’-CGTACACYTGTGATCGATGTG-3’ 56 [118]
X_Mcm7_R 5’- GTC TCC ACG TAT TCG CAT TCC-3’ 56 [118]
RPB1 gRPB1-A for 5’-GAKTGTCCKGGWCATTTTGG-3’ 54-56 [119]
fRPB1-C rev 5’-CCNGCDATNTCRTTRTCCATRTA-3’ 54-56 [119]
RPB1_MH_F 5’-ACGTCGCCGAGACCCHAARA-3’ 54-56 This study
RPB2 RPB2-6F 5’-TGGGGKWTGGTYTGYCCTGC-3’ 50-56 [120]
fRRPB2-7cR 5’-CCCATRGCTTGYTTRCCCAT-3’ 50-56 [120]
RPB2_MH_F 5’-ACAGTCGGTACWCCCAGYGAGCC-3’ 50-56 This study
RPB2_MH_R 5’-TGCCCATAGCCGATTGGTAYGTATT-3’ 50-56 This study

Leavitt et al.

Leavitt et al. BMC Evolutionary Biology 2012 12:176   doi:10.1186/1471-2148-12-176

Open Data