Expression of mouse PYHIN genes in perfused C57BL/6 mouse tissues (a), in various C57BL/6 mouse immune cells – splenic T and B cells, BMM and thioglycollate elicited peritoneal macrophages (TEPM) (b), and in BMM from three different mouse strains (c). Real time PCR results for each gene were normalised to the average of four housekeeping genes, which was found to give a relatively stable signal between tissues. Primers were tested to ensure lack of significant amplification of non-target PYHIN cDNAs. Results shown are the mean and range derived from duplicate RNA preparations.
Cridland et al. BMC Evolutionary Biology 2012 12:140 doi:10.1186/1471-2148-12-140