Evidence for a divergence in function between two glucocorticoid receptors from a basal teleost
1 Nutritional Sciences Research Division, King’s College London, Franklin Wilkins Building, 150 Stamford Street, London SE1 9NH, UK
2 Institute of Aquaculture, University of Stirling, Stirling, FK9 4LA, UK
BMC Evolutionary Biology 2012, 12:137 doi:10.1186/1471-2148-12-137Published: 3 August 2012
Additional file 1 :
The supplementary data files contain an alignment of the C-terminus of the rainbow trout andPantodon buchholziGRs (Figure S1) as well as an alignment of the region of the teleost GR E-domains that possesses the isoleucine/phenylanaline mutation that is proposed by Arterbery et al. [] to enhance mineralocorticoid selectivity in one of the duplicated GRs in the plain midshipman (Figure S2). In addition, Table S1 and S2 provide information on the modelling of the docking of cortisol and 11-deoxycortisol in the hormone binding pocket of Acipenser ruthenus and Pantodon buchholzi GRs. Figure S3 aligns the E-domain of the Acipenser ruthenus and Pantodon buchholzi GRs and highlights those amino acids that are predicted to interact with the ligand based on our modelling exercise and matches those to the amino acids predicted to interact with dexamethasone in the mammalian GR .
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