## Table 2 |
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Accessions of and DryopterisPolystichum included in this study |
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Species |
Ploidy level (reference) |
Collection locality |
# gapCp copies |
# pgiC copies |
plastid? |
Padre? |

D. abbreviata |
2x [51] | Turkey | — | ✓ (1) | ✓ | ✓ |

D. affinis |
2x, 3x [56] | Spain | ✓ (2) | ** | ✓ | — |

D. alpestris |
2x [57] | China | ✓ (1) | ✓ (1) | ✓ | ✓ |

D. antarctica |
unknown | Reunion | ✓ (2) | ✓ (3) | ✓ | — |

D. aquilinoides |
unknown | Reunion | ✓ (2) | ✓ (2) | ✓ | — |

D. arguta 1 |
2x [58] | Oregon | ✓ (1) | ✓ (1) | ✓ | ✓ |

D. arguta 2 |
2x [58] | Oregon | ✓ (1) | ✓ (1) | ✓ | — |

D. assimilis^{a} |
2x [59] | Russia | — | ✓ (1) | ✓ | ✓ |

D. austriaca^{b} |
4x [40] | Caucasus region | — | ✓ (1) | ✓ | — |

D. campyloptera 1 |
4x [23] | North Carolina | ✓ (2) | ✓ (2) | ✓ | ✓ |

D. campyloptera 2 |
4x [23] | North Carolina | ✓ (2) | ✓ (2) | ✓ | — |

D. carthusiana 1 |
4x; [51] | New York | ✓ (2) | ✓ (2) | ✓ | ✓ |

D. carthusiana 2 |
4x; [51] | Washington | ✓ (2) | ✓ (2) | ✓ | — |

D. carthusiana 3 |
4x; [51] | Wisconsin | ✓ (2) | ✓ (2) | ✓ | — |

D. carthusiana 4 |
4x; [51] | Wisconsin | — | ✓ (2) | ✓ | — |

D. caucasica |
2x [35] | Turkey | ✓ (2) | ✓ (2) | ✓ | — |

D. celsa 1 |
4x [24] | Georgia | ✓ (1) | ✓ (2) | ✓ | ✓ |

D. celsa 2 |
4x [24] | South Carolina | ✓ (2) | — | ✓ | — |

D. celsa 3 |
4x [24] | Louisiana | ✓ (2) | — | ✓ | — |

D. chrysocoma |
2x [60] | Taiwan | ✓ (1) | ✓ (3) | ✓ | — |

D. clintoniana 1 |
6x [24] | New York | ✓ (3) | ✓ (3) | ✓ | ✓ |

D. clintoniana 2 |
6x [24] | New York | ✓ (3) | — | ✓ | — |

D. crispifolia |
4x [59] | BPSSE; W. Europe | — | — | ✓ | — |

D. cristata 1 |
4x; [51] | South Carolina | ✓ (2) | ✓ (2) | ✓ | ✓ |

D. cristata 2 |
4x; [51] | Iowa | ✓ (2) | — | ✓ | — |

D. cristata 3 |
4x; [51] | Pennsylvania | ✓ (2) | — | ✓ | — |

D. cristata 4 |
4x; [51] | Wisconsin | ✓ (1) | — | ✓ | — |

D. cristata 5 |
4x; [51] | New York | ✓ (1) | — | ✓ | — |

D. cristata 6 |
4x; [51] | Michigan | ✓ (2) | — | ✓ | — |

D. cristata 7 |
4x; [51] | Michigan | ✓ (2) | — | ✓ | — |

D. dilatata 1 |
4x; [51] | Germany | ✓ (2) | ✓ (2) | ✓ | — |

D. dilatata 2 |
4x; [51] | Italy | ✓ (1) | — | ✓ | — |

D. dilatata 3 |
4x; [51] | France | — | ✓ (2) | ✓ | — |

D. expansa 1 |
2x [58] | British Columbia | ✓ (1) | ✓ (1) | ✓ | ✓ |

D. expansa 2 |
2x [58] | Washington | ✓ (1) | ✓ (1) | ✓ | — |

D. expansa 3 |
2x [58] | Oregon | ✓ (1) | ✓ (1) | ✓ | — |

D. expansa 4 |
2x [58] | Washington | — | ✓ (1) | ✓ | — |

D. filix-mas 1 |
4x; [51] | British Columbia | ✓ (2) | ✓ (1) | ✓ | — |

D. filix-mas 2 |
4x; [51] | Washington | ✓ (2) | ✓ (2) | ✓ | ✓ |

D. fragrans 1 |
2x [58] | Michigan | ✓ (1) | ✓ (1) | ✓ | ✓ |

D. fragrans 2 |
2x; [51] | Wisconsin | ✓ (1) | ✓ (1) | ✓ | — |

D. futura |
2x [61] | Guatemala | — | ✓ (3) | ✓ | — |

D. goldiana 1 |
2x [22] | North Carolina | ✓ (1) | ✓ (1) | ✓ | ✓ |

D. goldiana 2 |
2x [22] | Wisconsin | ✓ (1) | ✓ (1) | ✓ | — |

D. goldiana 3 |
2x [22] | New York | ✓ (1) | ✓ (1) | ✓ | — |

D. goldiana 4 |
2x [22] | New York | ✓ (1) | — | ✓ | — |

D. goldiana 5 |
2x [22] | Michigan | ✓ (1) | — | ✓ | — |

D. guanchica |
4x [59] | Cabildo of Tenerife | — | — | ✓ | — |

D. huberi |
unknown | Brazil | — | ✓ (2) | ✓ | — |

D. intermedia 1 |
2x [22] | North Carolina | ✓ (1) | ✓ (1) | ✓ | — |

D. intermedia 2 |
2x [22] | Wisconsin | ✓ (1) | ✓ (1) | ✓ | ✓ |

D. intermedia 3 |
2x [22] | New York | ✓ (1) | ✓ (1) | ✓ | — |

D. intermedia 4 |
2x [22] | New York | ✓ (1) | ✓ (1) | ✓ | — |

D. intermedia 5 |
2x [22] | New York | — | ✓ (1) | ✓ | — |

D. intermedia 6 |
2x [22] | Michigan | ✓ (1) | — | ✓ | — |

D. ludoviciana A208 |
2x [22] | South Carolina | — | * | — | — |

D. ludoviciana 1 |
2x [22] | South Carolina | ✓ (1) | ✓ (1) | ✓ | ✓ |

D. ludoviciana 2 |
2x [22] | Alabama | ✓ (1) | ✓ (1) | ✓ | — |

D. ludoviciana 3 |
2x [22] | Alabama | — | ✓ (1) | ✓ | — |

D. ludoviciana 4 |
2x [22] | Alabama | — | ✓ (1) | ✓ | — |

D. ludoviciana 5 |
2x [22] | Alabama | ✓ (1) | — | ✓ | — |

D. marginalis |
2x [58] | South Carolina | ✓ (1) | ✓ (1) | ✓ | ✓ |

D. monticola |
unknown | Japan | — | ✓ (2) | ✓ | — |

D. muenchii 1 |
3x [62] | Mexico | ✓ (3) | ✓ (3) | ✓ | — |

D. muenchii 2 |
3x [62] | Mexico | ✓ (3) | ✓ (3) | ✓ | — |

D. oligodonta |
2x [59] | Cabildo of Tenerife | — | ✓ (1) | ✓ | ✓ |

D. oreades |
2x [63] | Caucasus region | — | ** | ✓ | ✓ |

D. pallida |
2x [58] | AFSSE; W. Europe | — | ✓ (1) | ✓ | ✓ |

D. remota 1 |
3x; [51] | Germany | ✓ (2) | — | ✓ | — |

D. remota 2 |
3x; [51] | Asia | — | ✓ (2) | ✓ | — |

D. scottii |
4x [60] | Taiwan | — | ✓ (2) | ✓ | — |

D. tokyoensis |
2x [58] | Japan | ✓ (1) | ✓ (1) | ✓ | ✓ |

Polystichum andersonii |
Washington | — | ✓ (1) | ✓ | — | |

Polystichum munitum |
Washington | ✓ (1) | — | ✓ | ✓ |

Numbers after species names indicate that multiple accessions of that species were
included. Collection locations are given, and inclusion in plastid, *pgiC, gapCp*, and PADRE datasets is indicated. AFSSE and BPSSE indicate species that were obtained
as spores from the American Fern Society Spore Exchange and British Pteridological
Society Spore Exchange, respectively. These spores were germinated and grown for use
by Geiger and Ranker
[64], and DNA material later provided to us. Number of copies of *gapCp* and *pgiC* are given in parentheses if we successfully sequenced that region for an accession.
Ploidy level is given when known, with references. See Additional file
1: Table
1 for voucher information.

* The *pgiC* sequence for *D. ludoviciana* A208 was obtained from Genbank, and we included it in the current study as it was
the basis for a recent rejection of the “semicristata” hypothesis
[49].

** We were unable to sequence *pgiC* from our *D. affinis* and *D. oreades* accessions, and so obtained *pgiC* sequences for these taxa from Genbank. These sequences are therefore not from the
same accessions as the *gapCp* and plastid sequences.

^{a} synonymous with *D. expansa*.

^{b} synonymous with *D. dilatata*.

Sessa * et al.*

Sessa * et al.* *BMC Evolutionary Biology* 2012 **12**:104 doi:10.1186/1471-2148-12-104