Migration assay. A) CAL 27 cells, stable clones transfected with pcDNA3 vector (K0), pcDNA3FLAG/Nme1 (K1 and K2) and pcDNA3FLAG/NmeGp1Sd (S1 and S2) were tested for migration potential in Boyden chambers. The cells were stained with crystal violet. The images were recorded by inverted light microscope. The K and S clones clearly show lower migratory potential compared to untransfected CAL 27 and K0 control cells. Bar = 50 μm. B) The results are presented as a relative number of migrated cells (± SD) compared to CAL 27. The results were produced by ImageJ program measuring the membrane area covered with migrated cells. The results show that S1 and S2 clones as well as K1 and K2 exhibit diminished migration potential compared to CAL 27 and CAL 27 transfected with "empty vector" (K0).
Perina et al. BMC Evolutionary Biology 2011 11:87 doi:10.1186/1471-2148-11-87