DNA-binding activity of the NmeGp1Sd protein. Human Nme1 and Nme2 proteins were used as control. The reaction was performed in buffer containing 40 mM Tris-acetate (pH 7.5) and 12 mM MgCl2, 30 nM of single-stranded circular (ssc) DNA from bacteriophage ϕX174 and purified NmeGp1Sd protein as indicated. 40 mM Tris-acetate (pH 7.5) containing 50% glycerol and 0.01% bromophenol blue was added before the products were analyzed in 0.6% agarose gel.
Perina et al. BMC Evolutionary Biology 2011 11:87 doi:10.1186/1471-2148-11-87