Table 3

Plasmid standard curve values

Plasmid::gene of interest

Linear Equation

Amplification Efficiency (%)

r-squared values (Rsq)

Primary survey

A180

Y = -4.976*Log(X) + 56.76

58.8

0.979

P180

Y = -4.539*Log(X) + 51.69

66.1

0.981

S180r

Y = -3.494*Log(X) + 43.42

83.8

0.993

S180

Y = -3.998*Log(X) + 51.22

77.9

0.986

RH2-1

Y = -4.141*Log(X) + 52.61

74.4

0.995

RH2-2

Y = -3.846*Log(X) + 41.51

82.0

0.996

SWS2A

Y = -3.881*Log(X) + 43.15

81.0

0.942

SWS2B

Y = -4.142*Log(X) + 49.30

74.4

0.993

SWS1

Y = -3.378*Log(X) + 38.49

97.7

0.998

RH1

Y = -4.036*Log(X) + 39.05

76.9

0.990

COI

Y = -3.828*Log(X) + 41.05

82.5

0.991

B-actin

Y = -3.402*Log(X) + 38.67

96.8

0.996

Myosin-HC

Y = -3.651*Log(X) + 39.80

87.9

0.999

Secondary survey

COI

Y = -4.132*Log(X) + 47.75

74.6

0.957

B-actin

Y = -3.861*Log(X) + 42.84

81.6

0.973

Myosin-HC

Y = -4.217*Log(X) + 50.74

72.6

0.988

A180

Y = -3.774*Log(X) + 43.27

84.1

0.998

S180

Y = -4.184*Log(X) + 51.10

73.4

0.984

Data shown are from triplicate RT-qPCR reactions run in parallel with guppy cDNA samples, and were used to determine opsin- or reference-gene transcript copy number.

Laver and Taylor BMC Evolutionary Biology 2011 11:81   doi:10.1186/1471-2148-11-81