Table 2 |
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|
Oligonucleotide primers and sequences |
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|
Visual opsin genea, reference geneb, or plasmidc |
Primer Name |
Primer Sequence (5'→3') |
Amplicon size (bp) and GenBank accession number |
Primers designed against GenBank accession number or reference |
|
|
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|
LWS A180a |
A180SpecExon2 |
GGGTTTACAACGTCTCCACTC |
(1017) |
[8] |
|
A1803'UTR-DS |
CAATGCAACTATGTTCATT |
|||
|
LWS P180a |
pExon2 |
GATGGGTTTACGATGTCGCAACGG |
(1020) |
[8] |
|
LWS2 IntRev |
CAGTCCCGGCAGTAATAACAAAC |
|||
|
LWS S180a |
a/sExon2 |
GATGGGTTTACAACGTCTCCACAC |
1032 |
[8] |
|
LWS1 IntRev |
CATTTGCCATAAAGTTTCCGTTTATC |
|||
|
LWS S180ra |
S180rEx1-Ex2span-S |
GCAATCATACAAGAGATCC |
(1082) |
[35] |
|
S180r3'UTR-S |
ACACAAAACCTTCACTTTTAAGC |
|||
|
RH1a |
RH1-F |
ATGCATGGCTACTTTGTCC |
(538) |
|
|
RH1-S-R |
GTGTGAAGATGTACCAGGCCACAC |
|||
|
RH2-1a |
RH21-F |
CATGGTGGACCCAATGATCTA |
(827) |
|
|
RH21-R |
AGATAACAGGATTGTAAAGG |
|||
|
RH2-2a |
RH22-F |
CAGATCCATGGCAATTCAAA |
(738) |
|
|
RH22-R |
TAAAGAAAATCCACGCAGCA |
|||
|
SWS1a |
SWS1-F |
TGCAGGCGCTCTTCATGG |
(799) |
|
|
SWS1-R |
ATGAGCGGGTTGTAGAC |
|||
|
SWS2Ba |
SWS2B-F |
AGGGAGCCTGGGGCTTTTT |
(803) |
|
|
SWS2B-R |
TGGAGGCTTTTGAGACACAG |
|||
|
SWS2Aa |
SWS2A-F |
GGCACTTCCATCAACACC |
(480) |
|
|
SWS2A-R |
GAAGCAGAAAAGGAACATGACG |
|||
|
COIb* |
Cyt-c-F |
GAGCCCTTAAATGGGAGACC |
(445) |
|
|
Cyt-c-R |
GCTGCGAAAGCTTCTCAAAT |
|||
|
β-actin b |
β-actin-F |
GAAACCGGTTCCCTTAAAGC |
(439) |
|
|
β-actin-R |
GGGGTGTTGAAGGTCTCAAA |
|||
|
Myosin-HC b |
Myosin-HC-F |
ACACCAGCCTTCTGAACACC |
(491) |
|
|
Myosin-HC-R |
CTCGGCCTGTCTCTTGTAGG |
|||
|
pGEM-T c |
SP6 |
TATTTAGGTGACACTATAG |
MCS (141) + Insert |
Promega® |
|
pGEM-T c |
T7 |
TAATACGACTCACTATAGGG |
||
|
|
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|
*cytochrome c oxidase subunit 1. |
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|
Laver and Taylor BMC Evolutionary Biology 2011 11:81 doi:10.1186/1471-2148-11-81 |
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