Table 1

Guppy housing and rearing

Guppy (n-value) life cycle stage when

sequestered in light-dark cycle

Number of offspring

in brood

Age at time of

samplinga

Total RNA [ng/μL] b and purity (A260/280)

from pooled c left eyes

Total RNA [ng/μL] and purity (A260/280)

from pooled right eyes


Primary survey


Gravid female (1)

9 (brood-1)

1-month

juveniles d

272.9 (2.08)

554.9 (2.09)

Gravid female (1)

12 (brood-2)

1-month

juveniles

396.4 (2.10)

429.2 (2.07)

Gravid female (1)

8

2-month

juveniles

429.8 (2.10)

470.5 (2.11)

Males (10)

-

Adult

535.0 (2.07)

672.0 (2.10)

Females (6)

-

Adult

441.6 (2.14)

488.3 (2.10)


Secondary survey


Males1 (5)

-

Adult

544.8 (2.10)

Males2 (5)

-

Adult

555.6 (2.07)

Males3 (5)

-

Adult

250.8 (2.09)

Males4 (5)

-

Adult

463.8 (2.03)

Females1 (5)

-

Adult

803.4 (2.10)

Females2 (5)

-

Adult

776.3 (2.10)

Females3 (5)

-

Adult

158.8 (2.11)

Females4 (5)

-

Adult

753.2 (2.12)


a Euthanasia, followed by immediate eye excision, RNA extraction, and cDNA synthesis.

b Concentration and purity are mean of duplicate measurements within 5ng/μl of each other.

c Each RNA pool corresponds to 1 of the 18 cDNA samples subsequently generated.

d 1 month = 28 days.

Note both 1- and 2-month-old juveniles are generally sexually indistinguishable (i.e., not sexually dimorphic); for brevity, the designations 'male' and 'female' are only assigned to adults in this study. Males and females were exposed to each other (i.e., kept in the same aquaria). Male and female guppy numbers (subscripts 1-4) identify different groups.

Laver and Taylor BMC Evolutionary Biology 2011 11:81   doi:10.1186/1471-2148-11-81

Open Data