Additional file 4.
Reference gene copy number for primary (A) and secondary (B) surveys. Transcript copy number of COI, β-actin, and Myosin-HC reference genes determined by RT-qPCR analysis of guppy cDNA samples. (L) and (R) denote left and right eyes, respectively. Error bars (±S.E.M. of triplicate reactions) are shown for all samples, though most are too small to see.
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Laver and Taylor BMC Evolutionary Biology 2011 11:81 doi:10.1186/1471-2148-11-81