Relative abundance of putative cone opsin transcripts for: (A) one- and two-month old juvenile (1MOJ and 2MOJ, respectively) and adult (male and female) cDNA samples derived from left (L) and right (R) eye mRNA pools isolated from 6-12 individuals for each age or sex (see primary survey, Table 1). Error bars (±S.E.M. of triplicate reactions) are shown for all samples. (B) Inset magnifying A180 and S180 transcript relative abundance levels from panel A. (C) cDNA from four groups (n = 4) of five individuals (left and right eyes pooled) for each sex (see secondary survey, Table 1) used to determine if the slight A180 and large S180 upregulation in females observed in panel B is real. A statistically significant, p < 0.005 (asterisks), difference was observed between males and females for both A180 and S180 expression (based on independent student's unpaired t-tests of data before calibration to female expression). Error bars (±S.E.M. of four biological replicates) are shown. Note 'percent' relative abundance could not be calculated as only two of the nine 'cone' opsin genes were assayed. (A-C) Opsin transcript copy number was normalized to the transcript copy number (geometric mean) of three reference genes (asterisk): COI, β-actin, and Myosin-HC. Colors represent the λmax of each opsin (based on Wavelength to RGB v.1.0 software).
Laver and Taylor BMC Evolutionary Biology 2011 11:81 doi:10.1186/1471-2148-11-81