Table 2

Testing if four Lhr orthologs have hybrid male lethal activity

F1 Progeny Class

Number of progeny

UAS transgene in D. melanogaster mother a

Phenotype

Genotype

D. mel control cross b

D. sim Lhr1 cross c


P{UAS-mel\Lhr::Yfp}1

Red-eyed male

UAS/+; GAL4/+

and

278

134

+/+; GAL4/+

Orange-eyed male

UAS/+; +/+

148

107

White-eyed male

+/+; +/+

143

141


Total

569 n.s.

382***

P{UAS-mel\Lhr::Yfp}2

Red-eyed male

UAS/+; GAL4/+

and

221

334

+/+; GAL4/+

Orange-eyed male

UAS/+; +/+

93

8

White-eyed male

+/+; +/+

102

314


Total

416 n.s.

656***

P{UAS-mel\Lhr::Yfp}3

Red-eyed male

UAS/+; GAL4/+

and

282

74

+/+; GAL4/+

Orange-eyed male

UAS/+; +/+

162

2

White-eyed male

+/+; +/+

175

82


Total

619 n.s.

158***

Φ {UAS-sim\Lhr::Yfp}

Red-eyed male

UAS/+; GAL4/+

and

139

117

+/+; GAL4/+

Orange-eyed male

UAS/+; +/+

72

39

White-eyed male

+/+; +/+

77

107


Total

288 n.s.

223***

Φ {UAS-yak\Lhr::Yfp}

Red-eyed male

UAS/+; GAL4/+

and

83

346

+/+; GAL4/+

Orange-eyed male

UAS/+; +/+

37

55

White-eyed male

+/+; +/+

45

396


Total

165 n.s.

797***

Φ {UAS-vir\Lhr::Yfp}

Red-eyed male

UAS/+; GAL4/+

and

158

164

+/+; GAL4/+

Orange-eyed male

UAS/+; +/+

76

114

White-eyed male

+/+; +/+

86

112


Total

320 n.s.

390 n.s.


a - Females heterozygous for the indicated UAS transgene and for P{Act5C-GAL4}17bFO1 were crossed to either D. melanogaster w1118/Y (control cross) or to D. simulans Lhr1 males. For the first two sets of crosses the females were of genotype w/w; UAS/+; GAL4/+; for the remaining crosses the females were of genotype w/w; UAS +/GAL4 +.

b - For the control crosses the ratios of red eye: orange eye: white eye progeny were tested for deviation from a 2:1:1 ratio using a chi-square test. n.s. = p > 0.05

c - The ratios of red eye: orange eye: white eye for hybrid crosses were compared to control crosses using a 3 × 2 contingency table and chi-square test. *** = p < 1 × 10-3; n.s. = p > 0.05, 2 degrees of freedom.

Brideau and Barbash BMC Evolutionary Biology 2011 11:57   doi:10.1186/1471-2148-11-57

Open Data