HP1 immunoprecipitates with and binds directly to LHR. A) HP1 immunoprecipitates with LHR in extracts from Drosophila Kc embryonic tissue culture cells. Anti-MYC immunoprecipitates from wild-type Kc cells (control) and LHR-MYC expressing cells were analyzed by Western blot using anti-MYC and anti-HP1 antibodies. The percent of each sample loaded is shown (%). Protein that did not immunoprecipitate is shown in the flow through lane (FT). B) LHR binds to HP1 in an in vitro binding assay. In vitro synthesized LHR-HA and HP1 proteins were mixed and the resulting complexes were immunoprecipitated with anti-HA antibody and analyzed by Western blot using anti-HA and anti-HP1 antibodies. Control lane is sample immunoprecipitated from an in vitro synthesis extract without DNA from either gene added.
Brideau and Barbash BMC Evolutionary Biology 2011 11:57 doi:10.1186/1471-2148-11-57