Additional file 4.

Results from the test of substitutional saturation. Substitutional saturation of the sequences was tested using DAMBE software. The index of substitutional saturation is smaller than the estimated critical value irrespective of the symmetry of the tree. The sequences are therefore not saturated.

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Schirrmeister et al. BMC Evolutionary Biology 2011 11:45   doi:10.1186/1471-2148-11-45