Analysis of expression of Pla-Epo hybrid proteins in E. coli. Expression of the Pla-Epo-hybrid proteins was analyzed by Western blotting of whole cells with a mixture of anti-Pla, anti-Epo, and anti-Pla-loop antisera. The constructs are indicated with their plasmid names (see Table 1). Migration distance of the Pla isoforms are indicated; pre-Pla is the immature form of Pla, α-Pla and γ-Pla are mature Pla proteins with differing conformations, and β-Pla is the mature autocleaved form of Pla.
Haiko et al. BMC Evolutionary Biology 2011 11:43 doi:10.1186/1471-2148-11-43