Phylogenetic and molecular evolutionary analyses. (A) A phylogenetic tree based on the combined dsx nucleotide sequences encoding OD1 and OD2 domains in six dipteran families and two lepidopteran species. The consensus of six equally parsimonious trees (tree length = 757 and parsimony-informative characters = 206) and the neighbor-joining tree (417 total characters) obtained using the combined nucleotide sequences are shown with bootstrap support above branches (shown only when greater than 50%). Taxonomic relationships are indicated in the right margin of the trees. The topology was rooted with the dsx corresponding sequences from the lepidopteran B. mori and Danaus plexippus. (B) Comparison of dipteran female-specific dsx coding sequences and localization of OD1 and OD2 domains. Pairwise synonymous (dS) and non-synonymous (dN) substitution rates and the mean pairwise ratio (dN/dS) values are placed above the corresponding coding sequence.
Salvemini et al. BMC Evolutionary Biology 2011 11:41 doi:10.1186/1471-2148-11-41