Additional file 3.
Phylogenetic tree of microproteins from chordates using an alignment of protein sequences. The unrooted tree was constructed by aligning translated sequences homologous to Exons 3-4 of mouse Msmb and subjecting the alignment to the program Protpars (Phylip) followed by Drawtree. Most branch points do not yield significant bootstrap values using various methods (not shown), but sequences generally cluster into the three groups highlighted. Accordingly, the position of zebrafish usmh1 in the tree is unlikely to reflect its true phylogeny, as predicted by its conserved synteny.
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Frankenberg et al. BMC Evolutionary Biology 2011 11:373 doi:10.1186/1471-2148-11-373